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Jan 1, 1995 · This chapter describes the use of the bialaphos resistance gene as a selectable marker in plant transformation, as a screenable marker in tissue culture and plant breeding, and as a reporter gene in plant molecular biology. The bar gene is a part of the biosynthesis pathway of bialaphos.
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The bar gene is a part of the biosynthesis pathway of...
- Cloning of a Phosphinothricin N-acetyltransferase Gene From Streptomyces Viridochromogenes Tü494 and Its Expression in Streptomyces Lividans and Escherichia COLI
The black bar in the insert indicates the location of the...
- Characterization of Phosphinothricin Acetyltransferase and C-terminal Enzymatically Active Fusion Proteins
The bar gene has also served as a useful selectable and...
- Gene Transfer to Cereals
The challenge Gene transfer to plants is routine and...
- Recombinant DNA Methodology II
Section VI: Mutagenesis and Gene Disruption; Section VII:...
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We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants.
- Keerti S. Rathore, Vijay K. Chowdhury, Thomas K. Hodges
- 1993
Jan 1, 1992 · In this chapter we report the use of the bar gene as a selectable marker in plant transformation, as a screenable marker in tissue culture and plant breeding, and as a reporter gene in plant molecular biology.
- K. D'halluin, M. De Block, J. Denecke, J. Janssens, J. Leemans, A. Reynaerts, J. Botterman
- 1992
Aug 19, 2020 · Thus, in this study, we used Agrobacterium-mediated gene transfer to introduce the EPSPS gene (resistance to glyphosate) and the Bar gene (resistance to glufosinate) into castor 2129 (high stalk excellent restorer line) and CSR 181 (dwarf excellent restorer line).
- Huibo Zhao, Yong Zhao, Rui Luo, Lifeng Yang, Guorui Li, Jianjun Di, Mu Peng, Lili Li, Qi Wen, Xiaoti...
- 2020
The current study provides an efficient screening system for the transformed plant of S. miltiorrhiza harboring bar gene. To determine the most suitable phosphinothricin concentration for plant selection, non-transformed leaf discs were grown on selection media containing six different phosphinothricin concentrations (0, 0.2, 0.4, 0.6, 0.8, and ...
- Yu Liu, Shi Xin Yang, Yan Cheng, Dong Qing Liu, Yong Zhang, Ke Jun Deng, Xue Lian Zheng
- 2015
Apr 10, 2017 · In this study, an efficient Agrobacterium-mediated transformation system that uses the bar gene as a selection agent for sugarcane was developed for use on an industrial scale. A bar/Basta selection system was successfully used in this transformation system. The bar gene is a herbicide resistance gene, and Basta is a herbicide. By using this ...
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A gene which confers resistance to the herbicide bialaphos ( bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite.
