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1. insilico.ehu.es › PCRIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › PCR

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   In silico PCR amplification. PCR may be simulated against up-to-date sequenced prokaryotic genomes. This service allows a maximum of 2 mismatches between primers and template, so the stringency of in silico PCR must be consider high.

2. insilico.ehu.es › PCR › infoIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › PCR › info

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   PCR amplification. The aim of in silico PCR is to provide an easy way to obtain the theoretical PCR results we may expect from DNA, by using up to date sequenced bacterial genomes (including, optionally, plasmids when available).

3. insilico.ehu.esIn silico molecular biology experiments: PCR, multiplex-PCR,...

   insilico.ehu.es

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   Nov 29, 2023 · Theoretical PCR amplification, AFLP-PCR and restriction digest of complete bacterial genomes. genomic data from sequenced prokaryotes.

4. insilico.ehu.es › PCR › indexIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › PCR › index

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   In silico PCR amplification. Input primers in fasta format. Primer 1 1 5'--3' C Primer 2 1 5'--3' C. Migroorganism Include plasmids (if available) Allow mismatches, but in nucleotides in 3' end Maximum length of bands nucleotides. 1 Degenerated nucleotides are allowed; A+T+G+C must be 10 or more. Info. Suggestions are welcome ...

5. insilico.ehu.es › mini_tools › PCRIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › mini_tools › PCR

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   In silico PCR amplification. Sequence. Primer 1 1 5'--3' Primer 2 1 5'--3' Allow one mismatch 2 Maximum lenght of bands nucleotides. 1 A,T,G,C and N are allowed; A+T+G+C must be 10 or more. 2 One mismatch is allowed in any position of primers. Source code ...

6. insilico.ehu.es › PCR › indexIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › PCR › index

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   PCR amplification. Input primers in fasta format. Primer 1 1 5'- -3' C. Primer 2 1 5'- -3' C. Migroorganism. Include plasmids (if available) Allow mismatches, but in nucleotides in 3' end. Maximum length of bands. nucleotides.

7. insilico.ehu.es › PCR_virusIn silico PCR amplification against sequenced viruses - UPV/EHU

   insilico.ehu.es › PCR_virus

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   PCR amplification against sequenced viruses. 1783 sequences from 1421 completely sequenced viruses (last update: 2010/05/31) Primer 1 1 5'- -3' C.

8. insilico.ehu.es › T-RFLP › infoIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › T-RFLP › info

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   PCR amplification The selected primers will amplify a 328 bp fragment from 16S ribosomal RNA. In E. coli genome there are 7 copies of the target sequence, so 7 amplicons will be obtained. .

9. insilico.ehu.es › DNA_fingerprinting › infoIn silico PCR amplification - UPV/EHU

   insilico.ehu.es › DNA_fingerprinting › info

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   Different strategies may be used to label the PCR fragments: usa of at least one labelled primer, addition of labelled nucleotides during polymerization or labelling amplicons after amplification. Labelling of DNA may be by radioactive or non-radioactive methods.

10. insilico.ehu.es › PCR-RFLP › infoIn silico PCR amplification - UPV/EHU

    insilico.ehu.es › PCR-RFLP › info

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    This script will compute amplicons yielded by PCR amplification, and position of amplicons within genome and their length will be shown. In a second step, the amplicons will be digested with selected endonucleases, and length of resulting fragments will be calculated.