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Design primers or assays for PCR, qPCR, or sequencing (any species). Customization of ~45 parameters, allowing qPCR assay designs: With specific primer, probe, or amplicon criteria. Across a specified location. Design algorithm includes multiple checks to reduce primer-dimer formation.
Aug 24, 2015 · Simplify planning of your qPCR experiments using IDT free, online tools for oligonucleotide analysis and PCR primer design. This article provides an overview of our predesigned qPCR sequences and the basics of designing customized PCR primers and hydrolysis probes with the PrimerQuest™ Tool.
Oct 21, 2013 · For PCR primer design, IDT recommends that you aim for PCR primers between 18 and 30 bases; however, the most important considerations for primer design should be the T m value and on-target binding efficiency. Primers should also be free of strong secondary structures and self-complementarity.
Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization. Use NCBI BLAST™.
Custom primer design made easy. Design custom PCR primers with one of our free online SciTools™. Build custom assays, analyze oligo properties, and add sequences directly to your cart for quick, easy ordering. EXPLORE TOOLS.
IDT offers several free online tools for qPCR primer and probe design. The PrimeTime™ Predesigned qPCR Assays Design Tool, PrimerQuest™ Tool, OligoAnalyzer™ Tool, and RealTime PCR Tool guide you through identifying primer and probe sequences to make sure they are specific to your assay.
Sep 5, 2023 · The PrimerQuest™ Tool offers you the option to customize almost 50 different parameters that will lead to the most efficient primer design for your experiment. These customizable parameters let you select primer, probe, and amplicon criteria to target regions.
Meet IDT. We’re a global genomics advocate, creating custom solutions for research in CRISPR, NGS, qPCR, synthetic biology, and more.
Order IDT's single-stranded, duplexed, and pooled DNA oligos. Design oligos and PCR primers with quenchers, fluorophores, spacers, linkers, and modifications.
PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR ® Green (Thermo Fisher Scientific) or EvaGreen ® (Biotium) dyes. Predesigned sequences for human, mouse, or rat are designed with advanced bioinformatic and thermodynamic sequence analytics and for easy selection.
