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Cloning vectors are designed for efficient transfer of foreign DNA into the host. Expression vectors have efficient machinery for cloning and expression of foreign gene in the host system.
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.
Vectors can transfer donar DNA fragment with gene of interest to host cell (recipient) and are capable of replicating in the host cell. Cloning vectors include plasmids, bacteriophages, cosmids, phasmids, Bacterial Artificial Chromosomes (BACs) & Yeast Artificial Chromosomes (YACs).
1. Cloning Vectors: We use a cloning vec tor when our aim is to just obtain numer ous copies (clones) of our gene of interest (hence the name cloning vectors). These are mostly used in construction of gene libraries. A number of organisms can be used as sources for cloning vectors.
The different types of vectors available for cloning are plasmids, bacteriophages, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs) and mammalian artificial chromosomes (MACs).
Cloning Vectors. Genetic vectors are vehicles for delivering foreign DNA into recipient cells. In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed.
ecombinant DNA technology. In this unit, you will be studying about cloning. ectors used in eukaryotes. The three major types of vectors include Yeast, plants and animal vectors. In most molecular biology techniques plasmid present inside the E. coli.
