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What are the applications of animal cell culture?
What is animal cell culture?
What cells are used in animal cell culture?
What is cell culture?
Jun 26, 2020 · Introduction. Cell culture is the process by which human, animal, or insect cells are grown in a favorable artificial environment. The cells may be derived from multicellular eukaryotes, already established cell lines or established cell strains.
- Anju Verma, Megha Verma, Anchal Singh
- Animal Biotechnology. 2020 : 269-293.
- 10.1016/B978-0-12-811710-1.00012-4
- 2020
- Animal Cell Culture
- Based on The Life Span of Culture, The Cell Lines Are Categorized Into Two Types
- Common Cell Lines
- Methods
- Applications of Cell Line
A. Primary cell culture
This is the cell culture obtained straight from the host tissue cells. The cells dissociated from the parental tissue are grown on a suitable container, and the culture thus obtained is called primary cell culture. Such culture comprises mostly heterogeneous cells, and most cells divide only for a limited time. However, these cells are much similar to their parents. Depending on their origin, primary cells grow either as an adherent monolayer or in a suspension.
B. Secondary cell culture and cell line
When a primary culture is sub-cultured, it is known as secondary culture, cell line, or sub-clone. The process involves removing the growth media and disassociating the adhered cells (usually enzymatically). Sub-culturing primary cells into different divisions lead to the generation of cell lines. During the passage, cells with the highest growth capacity predominate, resulting in a degree of genotypic and phenotypic uniformity in the population. However, as they are sub-cultured serially, th...
Finite cell lines
The cell lines that go through a limited number of cell divisions with a limited life span are known as finite cell lines. The cells pass several times and then lose their ability to proliferate, a genetically determined event known as senescence. Cell lines derived from primary cultures of normal cells are finite cell lines.
Continuous cell lines
When a finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes a continuous cell line. Such transformation or mutationcan occur spontaneously, chemically, or virally induced or from the establishment of cell cultures from malignant tissue. Cell cultures prepared in this way can be sub-cultured and grown indefinitely as permanent cell lines and are immortal. These cells are less adherent, fast-growing, less fastidious in their nutritional requireme...
Human cell lines: 1. MCF-7 (breast cancer) 2. HL 60 (leukemia) 3. HeLa (human cervical cancer cells) Primates cell lines: Vero (African green monkey kidney epithelial cells) Cell strain Lineage of cells originating from the primary culture is called strain. These are either derived from a primary culture or a cell line by the positive selection or ...
Growth Requirements The culture media used for cell cultures are generally quite complex, and culture condition widely varies for each cell type. However, media generally include amino acids, vitamins, salts (maintain osmotic pressure), glucose, a bicarbonate buffer system (maintains a pH between 7.2 and 7.4), growth factors, hormones, O2 and CO2. ...
A. Vaccines Production One of the most essential uses of cell culture is in the research and production of vaccines. The ability to grow large amounts of virus in cell culture eventually led to the creation of the polio vaccine, and cells are still used today on a large scale to produce vaccines for many other diseases, like rabies, chickenpox, hep...
May 2, 2024 · Some of the areas where animal cell culture has found most applications include cancer research, vaccine production, and gene therapy. The growth of animal cells on artificial media is difficult than growing microorganisms on artificial media and thus, require more nutrients and growth factors.
Animal cell culture is described as the . in vitro. maintenance and proliferation of animal cells that will continue to grow outside the living organisms if supplied with appropriate nutrients and growth conditions. The process of growing cells under laboratory conditions is termed as Cell Culture. It is carried out . in vitro (within glass) as ...
Mar 8, 2022 · Animal cell culture technology involves the culture of animal cells in artificial media. In this technique, cells are removed from tissue or whole organism and are cultured in artificially controlled conditions.
Jan 1, 2014 · This chapter describes the basics of animal cell culture along with the most recent applications. The primary aim is to progressively guide students through fundamental areas and to demonstrate an understanding of basic concepts of cell culture as well as how to perform cell cultures and handle cell lines.
Jun 6, 2024 · The aim of this chapter is to introduce animal cell culture, covering its main advances over the years, its main applications, such as the use of animal cell culture for vaccine production, and to address the main challenges facing the technology.
