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Assertion (A): DNA ligase plays an essential role in recombinant DNA technology. Reason (R): The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.
Isolation of the vector and the eukaryotic gene-source DNA. V. Cloning of cells (and foreign DNA). Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?
Verified by Toppr. The recombinant DNA can multiply as many times as the copy number of the plasmid vector, thereby determining the yield of recombinant protein. So, higher the copy number of the plasmid vector, higher will be yield of recombinant protein. Therefore Copy number of plasmid is directly related to copy number of gene for coding ...
Statement 3 - The enzyme that cleaves phosphodiester bonds at specific sites within the DNA is endonuclease. Assertion (A): DNA ligase plays an essential role in recombinant DNA technology. Reason (R): The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.
A plasmid can be used as vector to deliver any piece of foreign DNA into the host organism. The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on the cut ends of the DNA molecules and joins their ends.
Solution. Verified by Toppr. Correct option is B) If the plasmid is digested using EcoRI at a single recognition site, it produces two sticky ends. Consider this like cutting a circular thread ( plasmid ) with a scissor ( EcoRI ) that creates two ends ( sticky ends here ).
In a plasmid vector there may be multiple cloning sites made up of different types of restriction sites but all of them will be repeated only once, because multiple copies of the same restriction site will digest the vector to produce various fragments of DNA (after restriction enzyme action) and the vector will fail to serve its function to produce a recombinant DNA.
The plasmid that is used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA or cloning vector or gene carrier. So, the correct answer is 'Its ability to carry a foreign gene'.
CUSIS An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA. helps in the selection of Transformants (2) Recombinants (3) Non-Transformants & 3 both The protein products of the following Bitoxin genes 27.
322 - Is a number given to distinguish this plasmid from others developed in the same laboratory. E coli cloning vector pBR 322 has restriction sites (HindIII, EcoRI, BamHl, SaII, PvuII, PstI, ClaI), ori (origin of replication) and antibiotic resistance genes (a m p R and t e t R). rop codes for the proteins involved in the replication of the ...
