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OligoAnalyzer is a primer analysis tool for oligonucleotides. Design and analyze DNA and RNA oligos for insight into behavior and properties.
Jun 15, 2012 · IDT offers a powerful analytical tool for determining the properties of oligo sequences—the OligoAnalyzer Tool. This tool calculates the physical characteristics of any oligo sequence, including length, GC content, melting temperature (T m ), molecular weight, extinction coefficient, and optical density.
To determine the extinction coefficient, you can analyze your sequence using IDT's free OligoAnalyzer™ Tool. Results from this analysis will also provide you with nmol/ OD 260 and µg/OD 260 values.
Nov 5, 2020 · Watch how easily and quickly you can predict melting temperature, dimers, hairpins, and more for any oligonucleotide design with IDT’s OligoAnalyzer™ Tool.
How do I use the OligoAnalyzer™ Tool to check for possible hairpins and dimers formed by my oligo? To do hairpin or dimer analysis, click on the appropriate option to the right of your sequence. For hairpin analysis, you can change the default concentrations provided to match your reaction conditions.
Integrated DNA Technologies | IDT
Read how to check your PCR primers using the OligoAnalyzer™ program to ensure there are no significant primer design issues.
You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research.
Sep 5, 2023 · To determine physical characteristics of your oligonucleotide sequences, including length, GC content, melting temperature (Tm), molecular weight, extinction coefficient, and optical density, IDT offers a powerful analytical tool called the OligoAnalyzer™ Tool. By simply entering your nucleotide sequence, including DNA, RNA, mixed bases, and ...
The basics of designing customized primers and probes at IDT. Be sure to read the section, "The PrimerQuest Tool in 4 steps".
