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Dec 30, 2015 · OligoCalc was designed to give researchers an easy tool for finding and comparing melting temperatures using more accurate calculations. For oligonucleotides between 8 and 40 nucleotides, the nearest neighbor method is the preferred method.
Oligo Calculator. Enter Oligo Sequence in Box. Length. Melting Temperature (Tm) °C. %GC content. Molecular Weight: daltons (g/M) OD of 1 is equal to nanoMolar. Required Tm= Known Tm + 16.6log (required salt concentration / 0.195M), where the Known Tm is calculated from 0.195M using the OligoCalculator.
For sequences less than 14 nucleotides the same formula as the basic calculation is use, with a salt concentration adjustment. Tm= (wA+xT)*2 + (yG+zC)*4 - log10(0.050) + 16.6*log10( [Na+]) where w,x,y,z are the number of the bases A,T,G,C in the sequence, respectively.
OligoAnalyzer™ Tool. Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m ), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization.
Apr 22, 2007 · OligoCalc has a familiar ‘calculator’ look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature.
Oligo Calculator. Enter Oligo Sequence in Box. Length. Melting Temperature (Tm) °C. %GC content. Molecular Weight: daltons (g/M) OD of 1 is equal to picoMolar. To use this calculator, you must be using Netscape 2.0 or later.
OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900,000 accesses of OligoCalc from nearly 200,000 distinct hosts, excluding search engines.
Enter Oligonucleotide Sequence Below. OD and Molecular Weight calculations are for single-stranded DNA or RNA. Nucleotide base codes. Reverse Complement Strand (5' to 3') is: 5' modification (if any) 3' modification (if any) select ss/ds and DNA or RNA.
Sequence. Salt concentration (mM) Oligo concentration (µM) DNA or RNA. Calculate Properties.
Sep 28, 2007 · OligoCalc provides a convenient web interface for calculating the physical properties of DNA and RNA oligonucleotides including melting temperature, molecular weight, %GC content and absorbance coefficient for a given oligonucleotide sequence.
