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  1. OligoEvaluator™ - Sequence Analysis. Enter No. of Sequences to be Analyzed: 1. Sequence (5' to 3') Type: * Only Linear. Length: bp. Base Annotations: DNA = A, T, G, C or [dA], [dT], [dG], [dC] Base Degeneracies = B, D, H, K, M, N, R, S, V, W, Y or [dB], [dD], [dH], [dK], [dM], [dN], [dR], [dS], [dV], [dW], [dY] LNA® = [+A], [+T], [+G], [+C]

  2. We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm calculator.

  3. We are pleased to offer OligoEvaluator, our online oligonucleotide sequence calculator. OligoEvaluator is easy to use: register or login, select DNA or RNA, paste your sequence, and click calculate. All reported properties are available for export to a convenient Excel template.

  4. ©2014 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and ...

  5. Enter and analyze oligonucleotide sequences with various modifications, secondary structures and BLAST search options.

  6. Eurofins Genomics' Oligo Analysis Tool is a multifunctional tool, which gives you the option of checking your oligos before you order them. It also facilitates the set up of experiments by calculating the adequate amounts and dilutions for your oligo solutions.

  7. Dec 30, 2015 · For oligonucleotides between 8 and 40 nucleotides, the nearest neighbor method is the preferred method. Note that the equations were developed using 14-20mers, so this method is the most accurate for oligonucleotides of this length.

  8. Multiple Primer Analyzer. For analyzing and comparing multiple primer sequences simultaneously. Write or paste your primer sequences to the input field (upper window). The analyzer accepts text and table format (can be copied from an Excel file, for example).

  9. OligoAnalyzer™ Tool. Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m ), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization.

  10. We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of T m.