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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Select the product group of the polymerase or kit you plan to use. Select the polymerase or kit from the list of products. If needed, modify the recommended primer concentration.
The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
Step 1. Select a Promega Primer. OR. Enter Oligo Sequence. Step 2. Primer Concentration (nM) Step 3. Set salt and Mg++ by selecting a product or entering concentration values. Promega Buffer Enter Values. Buffer Selection. Formula.
OligoAnalyzer™ Tool. Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m ), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization.
Click to view the instructions. * Please enter the primer sequence (direction is from 5' to 3', non-ATCG characters will be automatically filtered):
This tool calculates the Tm using the nearest-neighbor method based on the primer's nucleotide sequence. Please consider this as one of the parameters when designing primers. Tm is the temperature at which 50% of the double-stranded DNA dissociates into single-stranded DNA.
Tm calculator for PCR and qPCR primer and probe annealing temperature calculation. Specific for PCR Biosystems polymerases & mixes.
Tm Calculator calculates the melting temperature (Tm) of DNA primers based on either basic calculations or advanced base-stacking thermodynamics. It offers insights into primer characteristics, such as length, C+G content, and molecular weight, catering to both basic and detailed nucleic acid analysis.
Predicting melting temperature (Tm) is a simple way to quantify the melting point of DNA. This tool calculates Tm with the nearest-neighbor method with adjustments for laboratory conditions such as [Mg++] and sequence concentrations.
LNA-enhanced oligonucleotides have different melting properties from DNA oligonucleotides. To use this melting temperature prediction (Tm) tool, just enter your sequence below and press "calculate". DNA nucleotides are entered as A, C, T or G, and LNA nucleotides are written +A, +C, +T or +G.
