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1. www.sigmaaldrich.com › US › enOligoEvaluator™ for Tm Calculation & Primer Analysis

   www.sigmaaldrich.com › US › en

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   OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values. All reported properties are available for export to a convenient Excel template. Open Calculator. Compute Oligo Properties, Resuspension & Dilution. The OligoEvaluator™ consists of three modules.

2. oligoevaluator.comOligoEvaluator™ - Sequence Analysis

   oligoevaluator.com

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   OligoEvaluator™ - Sequence Analysis. Enter No. of Sequences to be Analyzed: 1. Sequence (5' to 3') Type: * Only Linear. Length: bp. Base Annotations: DNA = A, T, G, C or [dA], [dT], [dG], [dC] Base Degeneracies = B, D, H, K, M, N, R, S, V, W, Y or [dB], [dD], [dH], [dK], [dM], [dN], [dR], [dS], [dV], [dW], [dY] LNA® = [+A], [+T], [+G], [+C]

3. biotools.nubic.northwestern.eduOligoCalc: Oligonucleotide Properties Calculator

   biotools.nubic.northwestern.edu

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   Dec 30, 2015 · OligoCalc was designed to give researchers an easy tool for finding and comparing melting temperatures using more accurate calculations. For oligonucleotides between 8 and 40 nucleotides, the nearest neighbor method is the preferred method.

4. www.sigmaaldrich.com › HU › enOligoEvaluator™ for T - Sigma-Aldrich: Analytical, Biology ...

   www.sigmaaldrich.com › HU › en

   We are pleased to offer OligoEvaluator™, our online oligonucleotide sequence calculator that provides primer analysis values for PCR: OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values.

5. www.sigmaaldrich.com › PL › enOligoEvaluator™ for Tm Calculation & Primer Analysis

   www.sigmaaldrich.com › PL › en

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   OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values. All reported properties are available for export to a convenient Excel template. Open Calculator. Compute Oligo Properties, Resuspension & Dilution. The OligoEvaluator™ consists of three modules.

6. mcb.berkeley.edu › labs › krantzOligo Calculator - Molecular and Cell Biology

   mcb.berkeley.edu › labs › krantz

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   Oligo Calculator. Enter Oligo Sequence in Box. Length. Melting Temperature (Tm) °C. %GC content. Molecular Weight: daltons (g/M) OD of 1 is equal to nanoMolar. Required Tm= Known Tm + 16.6log (required salt concentration / 0.195M), where the Known Tm is calculated from 0.195M using the OligoCalculator.

7. People also ask

   What is oligocalc?

   • OligoCalc was designed to give researchers an easy tool for finding and comparing melting temperatures using more accurate calculations. For oligonucleotides between 8 and 40 nucleotides, the nearest neighbor method is the preferred method.

   OligoCalc: Oligonucleotide Properties Calculator

   biotools.nubic.northwestern.edu/OligoCalc.html

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   How do you calculate oligocalc DNA mw?

   • DNA Molecular Weight (typically for synthesized DNA oligonucleotides. The OligoCalc DNA MW calculations assume that there is not a 5' monophosphate) Anhydrous Molecular Weight = (A n x 313.21) + (T n x 304.2) + (C n x 289.18) + (G n x 329.21) - 61.96 A n, T n, C n, and G n are the number of each respective nucleotide within the polynucleotide.

   OligoCalc: Oligonucleotide Properties Calculator

   biotools.nubic.northwestern.edu/OligoCalc.html

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   Is oligocalc a localized version?

   • Localized versions of OligoCalc are available in Russian . None of the following melting temperature (Tm) calculations provide an adjustment for magnesium or manganese divalent cation concentration, although lack of these cations has been shown to adversely affect proper duplex formation. See Nakano et al, (1999) Nucleic Acids Res. 27 :2957-65.

   OligoCalc: Oligonucleotide Properties Calculator

   biotools.nubic.northwestern.edu/OligoCalc.html

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   Which method is most accurate for oligonucleotides?

   • For oligonucleotides between 8 and 40 nucleotides, the nearest neighbor method is the preferred method. Note that the equations were developed using 14-20mers, so this method is the most accurate for oligonucleotides of this length.

   OligoCalc: Oligonucleotide Properties Calculator

   biotools.nubic.northwestern.edu/OligoCalc.html

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8. pubmed.ncbi.nlm.nih.gov › 17452344OligoCalc: an online oligonucleotide properties calculator

   pubmed.ncbi.nlm.nih.gov › 17452344

   OligoCalc has a familiar 'calculator' look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature.