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The single cell gel electrophoresis assay ( SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell.
The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix.
Jun 27, 2006 · We present a procedure for the comet assay, a gel electrophoresis–based method that can be used to measure DNA damage in individual eukaryotic cells.
Jan 5, 2021 · The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals. In this review, we highlight advantages and limitations of this in vivo comet assay in a regulatory context.
Aug 13, 2015 · The comet assay is used to follow the accumulation of DNA breaks (repair intermediates) with time of incubation. Recently, Slyskova and colleagues were the first to apply the in vitro DNA repair assays for BER and NER successfully on human tissue samples; specifically, colorectal carcinoma biopsies (Slyskova et al., 2012, 2014 ).
The alkaline comet assay (single-cell gel electrophoresis) is a sensitive method that detects DNA strand breaks (SBs) and alkali-labile sites (ALS) in the nucleus of virtually all types of eukaryotic cells.
Jan 27, 2023 · The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human.
The comet assay (single cell gel electrophoresis) is the most common method for measuring DNA damage in eukaryotic cells or disaggregated tissues.
May 18, 2013 · The comet assay (single cell gel electrophoresis) is the most common method for measuring DNA damage in eukaryotic cells or disaggregated tissues.
The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated.
