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Primer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets.
Jun 18, 2012 · We present a new software tool called Primer-BLAST to alleviate the difficulty in designing target-specific primers. This tool combines BLAST with a global alignment algorithm to ensure a full primer-target alignment and is sensitive enough to detect targets that have a significant number of mismatches to primers.
NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. Fixed primers can be specified for the design of LAMP primers, and subsequent Loop primers are then designed based on LAMP primer selection.
Oct 14, 2022 · Primer-BLAST is a combination of Primer3, a popular primer designing tool, and NCBI BLAST. Primer-BLAST has two basic modes. It can: Design primers to specifically amplify a template sequence or a set of related templates from a background nucleic acid population (BLAST database). Identify potential targets in a background nucleic acid ...
Jun 18, 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.
Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers will bind to unspecific regions of the organism’s genome via the BLAST algorithm.
Mar 23, 2021 · Primer-BLAST allows for the construction of primers for qPCR where the user can specify the melting temperature, reduce the amount of self-priming, and span exon-exon junctions in order to avoid amplification of contaminating genomic DNA.
