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Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...
The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses).
Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
Jul 1, 2004 · Basic Local Alignment Search Tool (BLAST) is a sequence similarity search program that can be used via a web interface or as a stand-alone tool ( 1, 2 ). There are several types of BLAST to compare all combinations of nucleotide or protein queries with nucleotide or protein databases.
Local alignments algorithms (such as BLAST) are most often used. A global alignment should only be used on sequences that share significant similarity over most of their extents, and then it will sometimes return a better presentation.
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Mar 21, 2024 · BLASTp (Protein BLAST): compares one or more protein query sequences to a subject protein sequence or a database of protein sequences. This is useful when trying to identify a protein (see From sequence to protein and gene below ).
ElasticBLAST starts instances for you, provisions them with BLAST software and loads the BLAST database you requested. ElasticBLAST runs your batches on the instances, queuing up the work with cloud native scheduling software (Kubernetes on GCP and AWS Batch on AWS).
This document first introduces the BLAST databases available from NCBI (in Section 2). The actual guide (Section 3) divides BLAST searches into several categories according to the nature and size of the input query and the primary goal of the search.
