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Bradford assays are dye-binding assays for fast and simple protein quantification. Learn about the principles, features, and product highlights of different Bradford assay kits and reagents.
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] . It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins. Principle. Figure 1.
The Bradford assay is faster, involves fewer mixing steps, does not require heating, and gives a more stable colorimetric response, its response is prone to influence from non protein sources, particularly detergents, and becomes progressively more nonlinear at the high end of its useful protein concentration range.
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Learn how to use the Bradford reagent, a solution of Coomassie G-250 dye, to measure protein concentrations by spectroscopy. The assay is based on the color change of the dye from red to blue upon binding protein.
Learn how to quantify proteins using the Bradford method, based on the binding of Coomassie Brilliant Blue dye to protein samples. Find materials, protocols, and standard curves for this assay.
Learn how to use the Bradford reagent, a colorimetric assay for protein quantification, with Bio-Rad kits. Find out the compatibility, interference, and standards of the assay.
Learn how to use Coomassie Blue G250 dye to measure protein concentration in biological samples. The protocol describes the standard and microassay methods, the reagent preparation, and the protein standard.
