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Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at specific sequences. There are hundreds of different restriction enzymes, allowing scientists to target a wide variety of recognition sequences.
Mar 24, 2021 · Digesting a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Over 210 restriction enzymes are 100% active in rCutSmart™ Buffer, making double digestion simple.
Feb 6, 2019 · Restriction digestion is a targeted DNA fragmentation technique. It is widely used in genotyping, popularly known as RFLP (Restriction Fragment Length Polymorphism). It includes subsidiary techniques like PCR and gel electrophoresis for DNA amplification and analysis, respectively.
Restriction Enzyme Digestion. Product Listing Application Overview. Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together.
Protocol for restriction digestion of plasmid & insert, purification, and ligation NOTES: First quantify the plasmid (ideally by gel comparison, not nanodrop), and quantify the insert DNA (usually a column-purified PCR product; nanodrop is OK for this) then set up digests, as below.
This protocol is for restriction digest of plasmid DN A. To see the full abstract and additional r esources, please visit http://www.addgene.org/plasmid_protocols/restriction_digest/.
Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.
May 18, 2018 · A diagnostic restriction enzyme digest takes advantage of the fact that restriction enzymes cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly used to verify your plasmid.
Subcloning by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss how to move a cDNA from one plasmid to another. However, the same technique can be used to move promoters, selectable markers, or any other DNA element between plasmids.
Jul 30, 2018 · Restriction Digest Protocol. A specific protocol for single digestion using this restriction enzyme can be accessed using our free online tool, NEBcloner . Please note that NEBcloner will also provide detailed double digest protocols using this enzyme.
