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What is a cell culture technique?
What is animal cell culture?
What is included in the introductory guide to animal cell culture?
What cells are used in animal cell culture?
Jun 26, 2020 · Animal cell culture technology in today’s scenario has become indispensable in the field of life sciences, which provides a basis to study regulation, proliferation, and differentiation and to perform genetic manipulation. It requires specific technical skills to carry out successfully.
- Anju Verma, Megha Verma, Anchal Singh
- Animal Biotechnology. 2020 : 269-293.
- 10.1016/B978-0-12-811710-1.00012-4
- 2020
- What Is Animal Cell Culture?
- Primary Cell Culture
- Secondary Cell Culture
- Cell Lines
- Procedure Or Protocol of Animal Cell Culture
- Advantages of Animal Cell Culture
- Disadvantages of Animal Cell Culture
- References
Animal cell culture is a type of biotechnological technique where animal cellsare artificially grown in a favorable environment. 1. The cells used in animal cell culture are usually obtained from multicellular eukaryotes and their established cell lines. 2. Animal cell culture is a common and widely used technique for the isolation of cells and the...
Primary cell culture is the first culture obtained directly from animal tissue via mechanical and chemical disintegration or enzymatic methods.The cells of the primary cell culture are slow-growing cells that carry all the characteristics of the original tissue or cells.Since these cultures are obtained directly from the origin, they have the same number of chromosomes as the original cells.Primary cell cultures are performed in order to preserve and maintain the growth of cells on an artificial growth medium at a particular condition.Secondary cell cultures are obtained after the primary cell cultures are subsequently subcultured over a period of time in fresh culture media.The cells of the secondary cell cultures are long-lasting as these have a higher lifespan due to the availability of appropriate nutrients at regular intervals of time.Secondary cell cultures are favored over primary cell cultures as these are more readily available and are easy to grow and preserve.These are formed from the enzymatic treatment of the adherent cells followed by washing and resuspension of cells in particular volumes of fresh media.A cell line is a group of cells that are formed from the subculture of primary culture consisting of a pure culture of cells. Cell lines usually display functional features that are close to the primary cells, but the genotype and phenotype of the cells can be modified. A cell line consists of several cell lineages with similar or different phenoty...
1. Growth Conditions
1. Animal cell culture requires the use of specific culture media that are more complex and specific than the basic culture media used for microbial growth. 2. Some of the important basic components of the media are inorganic salts, nitrogen source, energy source, vitamins, fat and fat-soluble vitamins, growth factors, and hormones. In some cases, pH buffering systems and antibiotics are also added. 3. The temperature for the growth depends on the source of the cell as different organisms req...
2. Primary cell culture
1. Primary cell cultures are obtained from fresh tissues that are removed from the organs with the help of an aseptic razor. 2. In some cases, the cells are removed by the use of chemical disintegrators or proteolytic enzymes. 3. The cell suspension obtained is washed with buffering liquid in order to remove the proteolytic enzymes. 4. The cell suspension is poured onto a flat surface which can be a culture vessel or a sterile Petri plate. 5. The cells that can adhere to the base of the vesse...
3. Cell thawing
1. In the case of subsequent subcultures, the preserved cell culture might have to be used. 2. The water bath is heated to a temperature of 37°C, and the growth media where the cells are to be plated is warmed. 3. The warm medium is added to the culture vessel. The vial with the frozen cells is then placed in the water bath until thawed. 4. After thawing, the via is washed with 70% alcohol on the outside. The cell suspension is pipetted into the cell culture vessel and swirled gently to mix e...
The following are some of the advantages of animal cell culture; 1. Cell culture is superior to other similar biotechnological approaches as it allows the alteration of different physiological and physiobiological conditions like temperature, pH, and osmotic pressure. 2. Animal cell culture enables studies related to cell metabolism and understand ...
Even though animal cell culture has been used as a technologically advanced method, there are some disadvantages associated with this approach. 1. It is a specialized technique that requires trained personnel and aseptic conditions. The technique is an expensive process as it requires costly equipment. 2. The subsequent subculture of the cell cultu...
Kaur, Gurvinder, and Jannette M Dufour. “Cell lines: Valuable tools or useless artifacts.” Spermatogenesisvol. 2,1 (2012): 1-5. doi:10.4161/spmg.19885Yao, Tatsuma, and Yuta Asayama. “Animal-cell culture media: History, characteristics, and current issues.” Reproductive medicine and biologyvol. 16,2 99-117. 21 Mar. 2017, doi:10.1002/rmb2.12024Lodish H, Berk A, Zipursky SL, et al. Molecular Cell Biology. 4th edition. New York: W. H. Freeman; 2000. Section 6.2, Growth of Animal Cells in Culture. Available from: https://www.ncbi.nlm.nih.go...Hudu, Shuaibu Abdullahi et al. “Cell Culture, Technology: Enhancing the Culture of Diagnosing Human Diseases.” Journal of clinical and diagnostic research : JCDRvol. 10,3 (2016): DE01-5. doi:10.786...Animal cell culture is described as the . in vitro. maintenance and proliferation of animal cells that will continue to grow outside the living organisms if supplied with appropriate nutrients and growth conditions. The process of growing cells under laboratory conditions is termed as Cell Culture. It is carried out . in vitro (within glass) as ...
Learn how to culture ATCC animal cells with this guide that covers media, subculturing, cryopreservation, and contamination. Find product sheets, references, and best practices for working in a pandemic environment.
Feb 21, 2023 · Abstract. The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research.
- 10.3390/cells12050682
- 2023/03
- Cells. 2023 Mar; 12(5): 682.
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points.
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals.
