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2 days ago · Chromatography. Chromatography comprises of a varied set of versatile and widely used purification techniques that can be used to separate proteins based on various properties (Figure 3). Affinity chromatography exploits specific interactions between a protein and a ligand attached to a resin. The target protein binds to the ligand, allowing ...
4 days ago · The affinity of the nsp9–nsp12 interaction was determined at between 150 and 500 μM (Figure 3F), revealing relatively weak binding. However, there are many examples in the literature where low-affinity interactions are of essential importance to biological processes, affording interactions that are dynamic, transient, and reversible [ 15 - 17 ].
2 days ago · immobilized-metal affinity chromatography (IMAC) and size- exclusion chromatography (SEC). OsHIPP43 interacted with Pwl2 with a calculated dissociation equilibrium constant (K d) of 191 nM, and high heat exchange upon binding, indicated by a change in enthalpy (ΔH) of −67.6 kcal/mol (Fig. 1). To assess whether B
4 days ago · Mixtures were then processed for Ni-NTA affinity chromatography, and co-purifying proteins were detected using SDS–PAGE. Molar excesses of Acrs are expressed relative to the number of Cas7 ...
4 days ago · Summary. The Rcs pathway is repressed by the inner membrane protein IgaA under non-stressed conditions. This repression is hypothesized to be relieved by the binding of the outer membrane-anchored RcsF to IgaA. However, the precise mechanism by which RcsF binding triggers the signaling remains unclear.
3 days ago · Among them, high-performance affinity chromatography (HPAC) proved to be a very useful and efficient technique to study intermolecular interactions between HSA and drugs . HPAC is advantageous as it features high precision and speed, easy handling, high reproducibility and applicability, and possibility of working in near-physiological conditions, among others [ 19 ].
1 day ago · TMCP2 sample was analyzed through reversed phase chromatography using a mass spectrometer as the detector (RP-MS). The analysis was carried out on a Vanquish Flex UHPLC equipped with a MAbPac RP 2.1 × 50 mm column (Thermo Scientific) performing a linear gradient from 20% B to 40% B in 8 min, where mobile phase A was 0.1% formic acid in MS-grade water and B was 0.1% formic acid in MS-grade acetonitrile.
