Search results
Jun 23, 2008 · This manual documents the BLAST (Basic Local Alignment Search Tool) command line applications developed at the National Center for Biotechnology Information (NCBI).
Enter the PCR template here (multiple templates are currently not supported). It is highly recommended to use refseq accession or GI (rather than the raw DNA sequence) whenever possible as this allows Primer-BLAST to better identify the template and thus perform better primer specificity checking.
Aug 6, 2024 · The Basic Local Alignment Search Tool (BLAST) finds regions of similarity between sequences. The program compares nucleotide or protein sequences and calculates the statistical significance of matches.
Use the Primer-BLAST tool to search with pair of primers. You can enter the forward and reverse primers in the primer input boxes on the form. Select the appropriate database and a taxonomic group (organism) in the ‘Primer Pair Specificity Checking Parameters’ section of the form and click the ‘Get Primers’ button.
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
The Job Dispatcher at EMBL-EBI offers free access to a range of bioinformatics tools and biological datasets through its web and programmatic interfaces.
The statistics of global sequence comparison The statistics of local sequence comparison Bit scores P-values Database searches The statistics of gapped alignments