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  1. 3 days ago · messenger RNA (mRNA), molecule in cells that carries codes from the DNA in the nucleus to the sites of protein synthesis in the cytoplasm (the ribosomes ). The molecule that would eventually become known as mRNA was first described in 1956 by scientists Elliot Volkin and Lazarus Astrachan. In addition to mRNA, there are two other major types of ...

  2. 2 days ago · Following RNA extraction, miR-21 and miR-548 expression levels were measured using SYBR green master mix and real-time quantitative PCR. Results: Our study revealed a notable rise in miR-21 and miR-548 expression levels in tumor tissue samples compared to marginal tissue samples.

  3. 5 days ago · Learn how to prepare RNA for gene expression analysis from cultured cells in 7 minutes. The Cells-to-CT 1-Step TaqMan Kit is a simple, quick alternative to traditional RNA extraction.

  4. 5 days ago · Following isolation, the RNA was quantified by NanoDrop spectrometry (Thermo Scientific, Waltham, MA, USA), and the integrity was confirmed by Agilent 2100 Bioanalyzer to generate RNA integrity number (RIN) value (which was generally above 9) and then stored at −80°C.

  5. 1 day ago · A comprehensive high-quality DNA and RNA extraction protocol for a range of cultivars and tissue types of the woody crop avocado. Plants , 11 ( 3 ) 242 . doi: 10.3390/plants11030242 Brosnan, Christopher A. and Mitter, Neena ( 2021 ).

  6. 2 days ago · RNA from PMM2-targeted cells (CRISPR-PMM2) and control plasmid-transfected cells (CRISPR-CTR) was collected to confirm the effective targeting of the PMM2 gene. We obtained three myoblast clones with a marked reduction in PMM2 mRNA expression compared with the corresponding CRISPR-CTR clones (10.7 ± 2.8 fold reduction at day 1; p < .0001).

  7. 5 days ago · 97 OSCC patients’ tissue samples were collected and subjected to RNA isolation, cDNA synthesis and quantitative real-time PCR to analyze PTCH1 and SMO expression. Protein expression was assessed through immunohistochemistry. Clinicopathological parameters were correlated with gene and protein expression.

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