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NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once.
NEBcutter is a web-based tool for identifying restriction enzyme recognition sites in a DNA sequence. It is provided by New England Biolabs (NEB), a leading manufacturer of enzymes for molecular biology research.
NEBcutter is a free online tool that helps you analyze and manipulate DNA sequences with restriction enzymes. Learn how to use it with video tutorials and examples.
NEBcutter is a free tool that is used for restriction enzyme analysis. There are many different ways to use the tool. In this video, you will learn how to find out which restriction enzymes can be used to cut your DNA sequence a specific number of times.
NEBcutter incorporates everything that is known about the methylation sensitivity of any of the enzymes displayed when they overlap say a Dam site or a Dcm site as well as CpG, EcoK and EcoB sites. When theoretical digests are performed the results include a comparison of the effects of methylation, highlighting the bands that shift.
May 10, 2005 · This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.
This tutorial explains how to use NEBcutter V3.0 to visualize a restriction enzyme digest on various gel systems by inputting your electrophoresis parameters.
