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The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques.
Lowry Method. The Lowry protein assay is based on the biuret reaction with additional steps and reagents to increase the sensitivity of detection. In the biuret reaction, copper interacts with four nitrogen atoms of peptides to form a cuprous complex. Lowry adds phosphomolybdic/phosphotungstic acid also known as Folin-Ciocalteu reagent.
The Lowry assay (1951) is an often-cited general use protein assay. For some time it was the method of choice for accurate protein determination for cell fractions, chromatography fractions, enzyme preparations, and so on.
Jan 1, 2009 · The Lowry procedure was used to measure the protein content (Waterborg, 2009 ). Using gel loading, a 50 µg protein sample was placed in a well, boiled for 5 min, electrophoresed using SDS-PAGE ...
Jan 1, 2018 · The modified Lowry method is a combination of the Biuret method, where copper ions react with the peptide bonds within the protein, and a reaction between Folin-Ciocalteu reagent and the ring structure on aromatic amino acids.
To estimate the protein using Lowry's method, The -CO-NH-bond (peptide) in polypeptide chain reacts with copper sulphate in an alkaline medium to give a blue colored complex as employed by...
1 Introduction. The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained ( 1 ).
