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Primer-BLAST is a web-based tool that allows you to design primers for PCR based on your template sequence and a database of reference sequences. You can adjust various parameters, such as primer length, melting temperature, exon/intron selection, and specificity checking, to optimize your primer design.
Primer-Blast helps users make primers that are specific to intended PCR target by screening them against user-selected database. It provides primer characteristics, such as melting temperature, GC%, self complementarity, and exception error, and allows users to re-search for specific primers by accepting some of the unintended targets.
BLAST (Basic Local Alignment Search Tool) compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
Jun 18, 2012 · We present a new software tool called Primer-BLAST to alleviate the difficulty in designing target-specific primers. This tool combines BLAST with a global alignment algorithm to ensure a full primer-target alignment and is sensitive enough to detect targets that have a significant number of mismatches to primers.
Learn how to use Primer BLAST, a tool that helps you design and evaluate PCR primers based on sequence databases. Follow the steps for different scenarios: one or both primer sequences, target template sequence or accession number.
Primer-BLAST uses Primer3 to design PCR primers and screens them against user-selected database to avoid non-specific amplifications. It is a web-based tool that requires primer sequence, target sequence, and database as input.
Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties. This tool is publicly available at http://www.ncbi.nlm.nih.gov/tools/primer-bl …
Try "re-search for specific primers" feature on Primer-BLAST result page. When only non-specific primers are found, the result page offers the option to re-search for specific primers.
Jun 28, 2017 · NCBI announces the retirement of e-PCR tool and recommends using Primer-BLAST for primer design and specificity checking. Primer-BLAST is a flexible and powerful tool that can design primers for any template sequence and search a user-selected database for primer locations.
A comprehensive guide to NCBI resources and tools for DNA and RNA research, including databases, downloads, submissions, and tools. Find information on genomic sequences, variations, annotations, pathogens, probes, and more.
