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May 11, 2012 · Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials.
A replica plating technique is described in which cultures are grown on individual agar discs in a Petri dish. Products of metabolism are confined to the discs and colony spreading is restricted.
In molecular biology and microbiology, replica plating is a technique in which one or more secondary Petri plates containing different solid ( agar -based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary plate (or ...
Jul 24, 2021 · Let’s look at two selection techniques that make this search possible. First, in both techniques, the chemical composition of the transfer plate is important for visual identification of the colonies being hunted. The use of a replica-plating device makes the identification possible.
Jan 1, 1989 · This chapter highlights replica plating of animal cells with emphasis on features of the technique that have emerged since its original description. Replica plating as used in microbial genetics permits the identification of relatively rare mutants without resorting to direct selections based on resistance to drugs and inhibitors.
Mutant selection – Replica plating technique, resistance selection method, substrate utilization method and carcinogenicity test Recombination – Transformation, conjugation and transduction
Jul 31, 2023 · They grow bacteria on master plate which is non selective, and they pick bacterial colonies from master plate on valvet cloath and innoculate them on two petri plates containing selective media.
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