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3. Replica-Plating Technique: Replica-plating technique is often used to isolate nutritional mutants (auxotrophs) as well as various other type of mutants, e.g., antibiotic resistant mutants. For convenience, if one wants to isolate nutritional mutants employing replica-plating techniques (Fig. 29.13), he is required to follow the following steps:
Luria SE, Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. [ PMC free article] [ PubMed] [ Google Scholar] NOVICK A, SZILARD L. Experiments with the Chemostat on spontaneous mutations of bacteria. Proc Natl Acad Sci U S A. 1950 Dec;36(12):708–719.
Mar 30, 2020 · Replica plating technique used to detect auxotrophic mutant (microorganisms are not capable to synthesize their own organic compound or food)
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- Rajni Karakoti
A replica plating method was developed to study ecology of microorganisms in soil. Precise placement of inocula and amendments at desired loci in sterile soil contained in petri plates were accomplished with a template. Subsequent growth and distribution of individual species, even when part of a mixed population, was measured by periodic transfer with an easily constructed replicator to agar plates of differing nutritional composition or containing selective inhibitors. The method is rapid ...
May 11, 2012 · Pour-plate technique. (A) A small volume of sample (between 0.1 to 1.0 ml) is dispensed aseptically into an empty but sterile Petri dish with using a 5.0 ml serological pipette. (B) Melted agar ...
Apr 13, 2020 · Replica plating is a technique in which one or more secondary Petri plates containing different solid selective growth media are inoculated with the same col...
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- BaaYo
2. Replica plating of the master plate is required to retrieve the target cones, which is cumbersome. 7.3.1.2 Chromogenic Method It is one of the most widely and commonly used techniques, where the recombinant clones can be easily distinguished from the nonrecombinants based on the color of the colony.
