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   What are the principles of fluorescence in situ hybridization (FISH)?

   • Figure 1: Principles of fluorescence in situ hybridization (FISH). (a) The basic elements of FISH are a DNA probe and a target sequence. (b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling, and PCR.

   Fluorescence In Situ Hybridization (FISH) | Learn Science at Scitable

   www.nature.com/scitable/topicpage/fluorescence-in-situ-hybridization-fish-327/

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   What makes fish a successful in situ hybridization?

   • The success of FISH, and all other methods of in situ hybridization, depends on the remarkable stability of the DNA double helix. In 1953, James Watson and Francis Crick described the extensive network of hydrogen bonds that hold together the two antiparallel strands in the DNA double helix (Watson & Crick, 1953).

   Fluorescence In Situ Hybridization (FISH) | Learn Science at Scitable

   www.nature.com/scitable/topicpage/fluorescence-in-situ-hybridization-fish-327/

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   How does fish hybridize chromosomal regions?

   • FISH is indeed a simple and robust hybridization technique that requires only two things- target sequence and specific probe. Steps are elaboratively explained here. Target selection is a critical step. Keep in mind that FISH can effectively locate chromosomal regions of more than several thousand basepairs. So select a target having this property.

   A Brief Introduction to (FISH) Fluorescence In Situ Hybridization

   geneticeducation.co.in/a-brief-introduction-to-fish-fluorescence-in-situ-hybridization/

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   What is in situ hybridization?

   • In this technique, the in situ hybridization is combined with flow cytometry for measurement of the telomeric signals from cells in suspension. The PNA-labeled telomere probes are used to visualize and measure the length of telomere repeats. This technique has been used in aging studies.

   Fluorescence In Situ Hybridization (FISH) and Its Applications

   www.ncbi.nlm.nih.gov/pmc/articles/PMC7122835/

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2. en.wikipedia.org › wiki › Fluorescence_in_situFluorescence in situ hybridization - Wikipedia

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   Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.

3. www.ncbi.nlm.nih.gov › pmc › articlesFluorescence In Situ Hybridization (FISH) and Its Applications

   www.ncbi.nlm.nih.gov › pmc › articles

   Fluorescence in situ hybridization (FISH) is the most convincing technique for locating the specific DNA sequences, diagnosis of genetic diseases, gene mapping, and identification of novel oncogenes or genetic aberrations contributing to various types of cancers.

   • Author: Abdul Rauf Shakoori
   • Published: 2017 Feb

   • DOI: 10.1007/978-81-322-3673-3_16

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5. geneticeducation.co.in › a-brief-introduction-toA Brief Introduction to (FISH) Fluorescence In Situ Hybridization

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   Sep 27, 2019 · “FISH- Fluorescence In Situ Hybridization allows the direct study of a sequence located on the chromosome. It has been used for insertion and selection studies. Let’s see what FISH is and how it works.”

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6. www.genome.gov › genetics-glossary › Fluorescence-InFluorescence In Situ Hybridization (FISH)

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   6 days ago · Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that allows the localization of a specific DNA sequence or an entire chromosome in a cell.

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     Fluorescence In Situ Hybridization (FISH) || Application of FISH || Clinical relevance of FISH

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8. www.nature.com › scitable › topicpageFluorescence In Situ Hybridization (FISH) | Learn Science at ...

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   FISH is a technique that uses fluorescent probes to locate specific DNA sequences on chromosomes. Learn how FISH works, how it differs from in situ hybridization, and how it is used to diagnose chromosomal abnormalities.

9. my.clevelandclinic.org › health › diagnosticsFluorescence in Situ Hybridization (FISH Test) - Cleveland Clinic

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   Apr 22, 2024 · FISH is a technique that uses fluorescent labels to mark DNA and see it under a microscope. It can help diagnose diseases caused by chromosomal abnormalities or gene mutations, especially in cancer.

10. www.nature.com › scitable › contentPrinciples of fluorescence in situ hybridization | Learn ...

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    FISH is a technique that uses labeled DNA probes to detect and localize specific sequences in chromosomes or cells. Learn the principles, methods, and applications of FISH, as well as the advantages and disadvantages of direct and indirect labeling.