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OligoCalc was designed to give researchers an easy tool for finding and comparing melting temperatures using more accurate calculations. For oligonucleotides between 8 and 40 nucleotides, the nearest neighbor method is the preferred method.
OligoAnalyzer™ Tool. Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m ), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization.
For sequences less than 14 nucleotides the same formula as the basic calculation is use, with a salt concentration adjustment. Tm= (wA+xT)*2 + (yG+zC)*4 - log10(0.050) + 16.6*log10( [Na+]) where w,x,y,z are the number of the bases A,T,G,C in the sequence, respectively.
Oligo Calculator. Enter Oligo Sequence in Box. Length. Melting Temperature (Tm) °C. %GC content. Molecular Weight: daltons (g/M) OD of 1 is equal to nanoMolar. Required Tm= Known Tm + 16.6log (required salt concentration / 0.195M), where the Known Tm is calculated from 0.195M using the OligoCalculator.
Oligo Calculator. Enter Oligo Sequence in Box. Length. Melting Temperature (Tm) °C. %GC content. Molecular Weight: daltons (g/M) OD of 1 is equal to picoMolar. To use this calculator, you must be using Netscape 2.0 or later.
Jul 1, 2007 · OligoCalc has a familiar ‘calculator’ look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature.
Sep 28, 2007 · OligoCalc provides a convenient web interface for calculating the physical properties of DNA and RNA oligonucleotides including melting temperature, molecular weight, %GC content and absorbance coefficient for a given oligonucleotide sequence.
