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  1. Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Nucleotide Blast

      Select the sequence database to run searches against. No...

    • Protein Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • Blast FAQ

      You may also enter an NCBI accession or GI number. BLAST...

  2. Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...

  3. NCBI is a resource for biomedical and genomic information, including BLAST, a tool for sequence alignment and analysis. Learn about NCBI's mission, organization, news, and popular resources.

  4. ElasticBLAST is a service that allows you to run BLAST searches on cloud platforms such as Google Cloud Platform or Amazon Web Services. It distributes your queries across multiple instances, scales resources as needed, and stores results in a cloud bucket.

  5. Learn how to use BLAST, a tool for comparing sequences and finding similarities, with this comprehensive guide. Find answers to frequently asked questions, access BLAST software and databases, and explore BLAST topics and tutorials.

  6. Local alignments algorithms (such as BLAST) are most often used. A global alignment should only be used on sequences that share significant similarity over most of their extents, and then it will sometimes return a better presentation.

  7. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses).

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