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Ongoing development is partly funded by institutional grant IUT34-11 from the Estonian Ministry of Education and Research and by Centre of Excellence in Genomics and Translational Medicine at University of Tartu (funding from EU ERDF). Primer3 was a complete re-implementation of an earlier program: Primer 0.5 ( ).
Primer3Plus picks primers from a DNA sequence using Primer3. This is the latest version straight from the developers with all the new features.
Primer3 is a program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers. PCR is used for many different goals.
There is a newer version of Primer3 available at http://primer3.ut.ee. Paste source sequence below (5'->3', string of ACGTNacgtn -- other letters treated as N -- numbers and blanks ignored). FASTA format ok.
Primer3 - Manual. CONTENTS. 1. COPYRIGHT AND LICENSE 2. INTRODUCTION 3. CITING PRIMER3 4. FAIR USE OF PRIMER3 5. DIFFERENCE BETWEEN PRIMER3 AND PRIMER3PLUS 6. CHANGES FROM VERSION 2.2.3 7. INSTALLATION INSTRUCTIONS - UNIX/LINUX 8. INSTALLATION INSTRUCTIONS - Mac OSX 9. INSTALLATION INSTRUCTIONS - WINDOWS 10. SYSTEM REQUIREMENTS 11.
Select primer pairs to detect the given template sequence. Optionally targets and included/excluded regions can be specified. Mark an included region to pick primers fixed at its the boundaries.
Primer3 - Web Interfaces. There are several available versions of the Primer3 web interface: Primer3Web and Primer3Plus. Primer3Web is the original web-interface to Primer3. Primer3Web was developed by Steve Rozen and Helen Skaletsky. Primer3Web is maintained by Triinu Koressaar and Maido Remm. Primer3Web at primer3.ut.ee.
Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl.
Jun 6, 2012 · Download Primer3 - PCR primer design tool for free. Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3").
This specifies the minimal number of bases that the primer must anneal to the template at 5' side (i.e., toward start of the primer) or 3' side (i.e., toward end of the primer) of the exon-exon junction.
