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  1. 11 hours ago · In addition, HASMCs were pre-treated with AG490 (10 μM, Santa Cruz Biotechnology, Santa Cruz, California, USA) for 1 h to inhibit the JAK/STAT signaling pathway. ... Then PVDF membranes were ...

  2. 11 hours ago · The membrane was washed three times in TBS-T for 10 min at room temperature, then incubated for 1 h with rabbit HRP-conjugated secondary antibody against the primary antibody (Santa cruz sc-2357) diluted in blocking buffer (1:20.000). The membrane was finally revealed using ECL chemiluminescence (Perkin Elmer NEL105001EA). Luciferase assay

  3. 11 hours ago · Lung parenchymal hypoxia has emerged as a cardinal feature of idiopathic pulmonary fibrosis (IPF). Hypoxia promotes cancer cell invasion and metastasis through signaling that is dependent upon the lysophosphatidic acid (LPA) receptor, LPA1 (LPAR1). Abundant data indicate that LPA1-dependent signaling also enhances lung fibrogenesis in IPF. We recently reported that fibroblasts isolated from the lungs of individuals with IPF have an increased capacity to form subcellular matrix-degradative ...

  4. 11 hours ago · After transferring the proteins to the PVDF (Immobilon ®-P Transfer Membranes; Cat. 88518, Millipore, Burlington, MA, USA) and being blocked with 5% non-fat dry milk in TBST, the blots were probed overnight at 4 °C with the following primary antibodies (Santa Cruz, Dallas, TX, USA): goat polyclonal antibodies for AT1R (Cat.sc-1173-G, 1:1000 dilution); rabbit polyclonal antibodies for AT2R (Cat.sc-9040, 1:1000 dilution) and MAS1 (Cat. sc-135063, 1:1000 dilution); and mouse monoclonal ...

  5. 11 hours ago · Seed longevity is a crucial trait for the seed industry and genetic resource preservation. To develop excellent cultivars with extended seed lifespans, it is important to understand the mechanism of keeping seed germinability long term and to find useful genetic resources as prospective breeding materials. This study was conducted to identify the best cultivars with a high and stable seed longevity trait in the germplasm of rice (Oryza sativa L.) and to analyze the correlation between seed ...

  6. 11 hours ago · 和光纯药WAKO 637-00653 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) — –