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6 hours ago · RegPrecise 3.0 was selected as a core resource on transcriptional regulation of the Department of Energy Systems Biol. Knowledgebase, an emerging software and data environment designed to enable researchers to collaboratively generate, test and share new hypotheses about gene and protein functions, perform large-scale analyses, and model interactions in microbes, plants, and their communities.
10 hours ago · Phylogenetic trees were constructed based on the 16S rRNA genes and a set of 20 validated bacterial core genes (VBCG), respectively, while a phylogenomic tree was constructed based on protein-coding genes. Phylogenetic analysis was performed utilizing MEGA version 11 software (Tamura et al. 2021) based on the nearly complete 16S rRNA gene ...
10 hours ago · The final dataset comprised 528 nucleotide-length sequences. The sequences were aligned using the ClustalW algorithm with MEGA 11 software, and the evolutionary history was inferred through maximum likelihood, employing the Tamura–Nei model . To account for potential variations in the evolutionary rates across sites, a discrete gamma ...
10 hours ago · Phylogenetic tree of the fungi isolated from mangrove sediment. The tree was created by the neighbor joining method using 600 bootstraps using MEGA 11 software. MG1–MG5 refer to the fungi selected to the tannery effluent treatment
10 hours ago · Annealing was carried out for 1 min at 50 °C followed by extension for 2 min at 72 °C and a final extension for 7 min at 72 °C. BLAST search was used to identify the isolate, and neighbor-joining method was used to construct phylogenetic tree in MEGA software (version 7.2). Screening for Efficient Production Media
10 hours ago · DNA sequence was aligned using blast database 64 and the retrieved related sequences was aligned, and phylogenetic tree was constructed with MEGA 11 65. Cultivation and secondary metabolites ...
10 hours ago · Based on average nucleotide identity and phylogenetic analysis, the eight strains were classified into six novel species and two novel strains. The reconstruction of the metabolic pathways indicated that all strains had a complete Entner–Doudoroff pathway, pentose phosphate pathway, and diverse pathways for amino acid metabolism, suggesting the presence of an optimized central carbon metabolism.
