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7 hours ago · This study aimed to investigate the effect of chemical-free two-stage hydrothermal and mechanical refining pretreatment on improving the sugar yields during enzymatic hydrolysis of forest residue biomass (FRB) and optimize the pretreatment conditions. Hot-water pretreatment experiments were performed using a central composite design for three variables: temperature (160–200 °C), time (10–20 min), and solid loading (10–20%). Hydrothermally pretreated biomass was subsequently pretreated ...
