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The Bradford assay was first described by Dr. Marion Bradford in 1976 and uses Coomassie G-250 dye in a colorimetric reagent for the detection and quantitation of total protein. Pierce Bradford Plus Protein Assays are modifications of the reagent first reported by Dr. Bradford.
The Bradford assay is faster, involves fewer mixing steps, does not require heating, and gives a more stable colorimetric response, its response is prone to influence from non protein sources, particularly detergents, and becomes progressively more nonlinear at the high end of its useful protein concentration range.
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] . It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins. Principle. Figure 1.
Bio-Rad's Bradford assays provide a simple and accurate method for determining protein concentrations. The binding of the Bradford reagent to proteins results in a color change which is measured with a spectrophotometer or a microplate reader. Choose the kit that meets your needs.
The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.
Assay reagent: dissolve 100 mg of Coomassie Blue G250 in 50 mL of 95% ethanol. The solution is then mixed with 100 mL of 85% phosphoric acid and made up to 1 L with distilled water (see Note 5). The reagent should be filtered through Whatman No. 1 filter paper and then stored in an amber bottle at room temperature. It is stable for several weeks.
The Bradford method is a quantitative protein assay method, based on the binding of a dye, Coomassie Brilliant Blue, to a protein sample, and comparing this binding to a standard curve generated by the reaction of known amounts of a standard protein, usually BSA.
The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to various concentrations of protein. The dye reagents are commonly purchased from Bio-Rad (Richmond, CA).
Get fast results with a Bradford protein assay. Premixed or 5X Bradford reagent, albumin (BSA) or globulin standards. Compatible with most buffers and reagents.
137 Citations. 3 Altmetric. Abstract. A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories.
