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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Select the product group of the polymerase or kit you plan to use. Select the polymerase or kit from the list of products. If needed, modify the recommended primer concentration.
Tm Calculator Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Jul 27, 2023 · Please use NEB’s Tm Calculator to determine the appropriate annealing temperature for your primer pair and NEB polymerase/buffer of interest. Unlike other calculators, the NEB Tm Calculator takes into consideration buffer components that affect melting temperatures and empirical observations when calculating the optimal annealing temperature.
NEB Tm Calculator is used to estimate an appropriate annealing temperature in NEB PCR products. The tool fields are: Product Group: select a value from Q5, Q5 Hot Start, Q5U Hot Start, OneTaq, OneTaq Hot Start, Hot Start Taq, Taq DNA Polymerase, LongAmp Taq, LongAmp Hot Start Taq, Hemo KlenTaq, Vent, Deep Vent, Master Mix, Phusion, Phusion Hot ...
Dec 13, 2013 · The NEB Tm Calculator should be used to determine the annealing temperature when using this enzyme. Typically, use a 10–30 second annealing step at 3°C above the T m of the lower T m primer. A temperature gradient can also be used to optimize the annealing temperature for each primer pair.
Jul 27, 2023 · Please use NEB’s Tm Calculator to determine the appropriate annealing temperature for your primer pair and NEB polymerase/buffer of interest. Unlike other calculators, the NEB Tm Calculator takes into consideration buffer components that affect melting temperatures and empirical observations when calculating the optimal annealing temperature.
Tm Calculator. Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry.
Looking for an annealing temperature calculator that uses the Wallace et al equation to calculate NEB TM online? Then you've come to the right place! Annealing temperature (Tm) is the temperature at which primers or primers bind or “anneal” (form hydrogen bonds) to complementary strands of DNA, allowing extension by the polymerase.
Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Instructions. Select the product group of the polymerase or kit you plan to use. Select the polymerase or kit from the list of products. If needed, modify the recommended primer concentration.
